ELISA

Examples of ELISA in the following topics:

• Enzyme-Linked Immunosorbent Assay (ELISA)

  • Enzyme-linked immunosorbent assay (ELISA) is a solid-phase enzyme immunoassay used to detect the presence of a substance in solution.
  • Enzyme-linked immunosorbent assay (ELISA) is a method of quantifying an antigen immobilized on a solid surface.
  • ELISA uses a specific antibody with a covalently coupled enzyme.
  • Several variations of ELISA, seen in , exist but the most commonly used method is the sandwich ELISA.
  • Describe how the Enzyme-linked immunosorbent assay (ELISA) can be used to detect and quantitate antigens, antibodies and allergens

• Immunoblot Procedures

  • Immunoblots are most often used in research settings and are usually performed to confirm results from ELISA or other immunoassays.

• The Future of Diagnostic Immunology

• Western Blots

  • Other related techniques include using antibodies to detect proteins in tissues and cells by immunostaining and enzyme-linked immunosorbent assay (ELISA).

• Arboviral Encephalitis

  • TBE and other arboviral encephalitis can be diagnosed through a combination of blood tests, particularly immunologic, serologic, and/or virologic techniques such as ELISA, complement fixation, polymerase chain reaction, Neutralization test, and Hemoagglutination Inhibition test.

• Chikungunya Fever

  • Serological diagnosis requires a larger amount of blood than the other methods, and uses an ELISA assay to measure chikungunya-specific IgM levels.

• Proteomics

  • For more quantitative determinations of protein amounts, techniques such as ELISAs can be used.

• Histoplasmosis

  • It can also be diagnosed by detection of antigens in blood or urine samples by ELISA or PCR.