Section 14
Cloning Techniques
Book
Version 6
By Boundless
By Boundless
Boundless Microbiology
Microbiology
by Boundless
6 concepts
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Putting Foreign DNA into Cells
The methods used to get DNA into cells are varied (e.g., transformation, transduction, transfection, and electroporation).
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Obtaining DNA
When cloning genomic DNA, the DNA to be cloned is extracted from the organism of interest.
Hosts for Cloning Vectors
The majority of molecular cloning experiments begin with a laboratory strain of the bacterium E. coli (Escherichia coli) as the host.
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Shuttle Vectors and Expression Vectors
An expression vector is generally a plasmid that is used to introduce a specific gene into a target cell.
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Bacteriophage Lambda as a Cloning Vector
Enterobacteria phage λ (lambda phage, coliphage λ) is a bacterial virus that infects the bacterial species Escherichia coli.
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Vectors for Genomic Cloning and Sequencing
In molecular biology, a vector is a DNA molecule used as a vehicle to transfer foreign genetic material into another cell.