The Steps of PCR
This illustrates a PCR reaction to demonstrate how amplification leads to the exponential growth of a short product flanked by the primers. 1. Denaturing at 96°C. 2. Annealing at 68°C. 3. Elongation at 72°C. The first cycle is complete. The two resulting DNA strands make up the template DNA for the next cycle, thus doubling the amount of DNA duplicated for each new cycle.
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