quantitation

(noun)

The process of quantitating.

Related Terms

Examples of quantitation in the following topics:

  • Multiplex and Real-Time PCR

    • Multiplex and real-time PCR are molecular techniques designed to amplify nucleic acid sequences in a quantitative manner.
    • Real-time polymerase chain (RT-PCR) reaction, also called quantitative real-time PCR (qRt-PCR) is used to amplify and quantify targeted DNA molecules.
    • The use of RT-PCR allows for both detection and quantitation of DNA sequences.
    • Ideally, a real-time multiplex PCR should be able to detect, differentiate, and provide a quantitative result for many different targets without a single target influencing the detection of one of the others (cross-talk) and without loss of sensitivity.
    • Since its first description in 1988 by Chamberlain et al, this method has been applied in many areas of DNA testing, including analyses of deletions, mutations, and polymorphisms, or quantitative assays and reverse transcription PCR.

  • Precipitation Reactions

    • Precipitation methods include double immunodiffusion (qualitative gel technique that determines the relationship between antigen and antibody), radial immunodiffusion (semi-quantitation of proteins by gel diffusion using antibody incorporated in agar), and electroimmunodiffusion (variation of the double immunodiffusion method reaction that uses an electric current to enhance the mobility of the reactants toward each other).

  • Immunoblot Procedures

    • Western blotting is a routine molecular biology method that can be used to semi-quantitatively compare protein levels between extracts.

  • Immunoassays for Disease

    • These biochemical and serological techniques are based on the detection and quantitation of antibodies generated against an infectious agent, a microbe, or non-microbial antigen.

  • Basic Microbiology

    • Although microbes were directly observed over three hundred years ago, the precise determination, quantitation, and description of its functions is far from complete, given the overwhelming diversity detected by genetic and culture-independent means.

  • Enzyme-Linked Immunosorbent Assay (ELISA)

    • Describe how the Enzyme-linked immunosorbent assay (ELISA) can be used to detect and quantitate antigens, antibodies and allergens

  • Chemical Assays, Radioisotopic Methods, and Microelectrodes

    • Within the field of microbiology, there are specific tests or assays utilized to quantitatively and qualitatively measure microorganism components.

  • Viral Roles in Ecosystems

    • Phage-ecological interactions therefore are quantitatively vast, with huge numbers of interactions.

  • Microarrays and the Transciptome

    • Sequencing is now being used instead of gene arrays to quantify DNA levels, at least semi-quantitatively.

  • X-Ray Diffraction Analysis

    • Further analysis involves structure refinement and quantitative phase using the general structure analysis system (GSAS), which ultimately leads to the identification of the amorphous or crystalline phase of a matter and helps construct its three dimensional atomic model .