Examples of logarithmic growth phase in the following topics:
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- While it is possible to make a long term stock from cells in the stationary phase, ideally your culture should be in logarithmic growth phase.
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- In autecological studies, bacterial growth in batch culture can be modeled with four different phases: lag phase, exponential or log phase, stationary phase, and death phase .
- The exponential phase (sometimes called the log phase or the logarithmic phase) is a period characterized by cell doubling.
- For this type of exponential growth, plotting the natural logarithm of cell number against time produces a straight line.
- This chart shows the logarithmic growth of bacteria.
- The phases of growth are labelled on top.
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- Microbial growth is an important measure in understanding microbes.
- Since there are limits on space, food, and other factors, actual growth never matches actual measured growth.
- This chart shows the logarithmic growth of bacteria.
- Note the Y-axis scale is logarithmic meaning that the number represents doubling.
- The phases of growth are labelled on top.
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- During lag phase, bacteria adapt themselves to growth conditions.
- Exponential phase (sometimes called the log or logarithmic phase) is a period characterized by cell doubling.
- In comparison to batch culture, bacteria are maintained in exponential growth phase, and the growth rate of the bacteria is known.
- Bacterial growth in batch culture can be modeled with four different phases: lag phase (A), exponential or log phase (B), stationary phase (C), and death phase (D).
- List the growth phases of microrganisms and the different types of growth media available to culture them
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- When a cell switches to the biofilm mode of growth, it undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated.
- Recent evidence has shown that one fatty acid messenger, cis-2-decenoic acid, is capable of inducing dispersion and inhibiting growth of biofilm colonies.
- For instance, the biofilm form of Pseudomonas aeruginosa has no greater resistance to antimicrobials than do stationary-phase planktonic cells, although when the biofilm is compared to logarithmic-phase planktonic cells, the biofilm does show greater resistance to antimicrobials.
- This resistance to antibiotics in both stationary phase cells and biofilms may be due to the presence of persister cells.
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- Bacterial growth is the division of one bacterium into two daughter cells in a process called binary fission.
- When Escherichia coli is exposed to a temperature drop from 37 to 10 degrees Celsius, a four to five hour lag phase occurs and then growth is resumed at a reduced rate.
- During the lag phase, the expression of around 13 proteins, which contain cold shock domains is increased two- to ten-fold.
- Bacterial growth in batch culture can be modeled with four different phases: (A) the lag phase, when the population stays roughly the same; (B) the exponential, or log, phase, when the population grows at an increasing rate; (C) the stationary phase, when population growth stagnates; and (D) the death phase, when bacteria begin to die off and the population decreases in size.
- Describe how the growth of bacteria is affected by temperature and how bacterial growth can be measured
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- Nutrients are necessary for microbial growth and play a vital role in the proper cultivation of microorganisms in the laboratory and for proper growth in their natural environments.
- The limiting factor or limiting nutrient effects and controls growth.
- When all nutrients and parameters are ideal and constant during the growth phase, this is regarded as a steady state: all requirements are present and microorganisms thrive.
- In industrial microbiology this concept is critical, as microbial growth and production is dictated by proper cellular growth and metabolism.
- Describe the role of nutrients in microbial growth and their culture in the lab
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- In organisms that require carbon fixation, the Calvin cycle is a means to obtain energy and necessary components for growth.
- The Calvin cycle can be divided into three major phases which include: Phase 1: carbon fixation; Phase 2: reduction; and Phase 3: regeneration of ribulose .
- Once 3-PGA is formed, one of two molecules formed continues into the reduction phase (phase 2).
- This energy is necessary for cellular growth and metabolic processes.
- Outline the function of the intermediates produced in the major phases of the Calvin Cycle
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- Bacterial growth follows three phases: the lag phase, the log phase, and the stationary phase.
- The measurement of an exponential bacterial growth curve in a batch culture was traditionally a part of the training of all microbiologists; the basic means requires bacterial enumeration (cell counting) by direct and individual (microscopic, flow cytometry), direct and bulk (biomass), indirect and individual (colony counting), or indirect and bulk (most probable number, turbidity , nutrient uptake) methods.
- This has made spectrophotometry the methods of choice for quick measurements of bacterial growth and related applications.
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- Bacterial metabolism can be classified into three major categories: the kind of energy used for growth, the carbon source, and the electron donors used for growth.
- Primary metabolites are involved in growth, development, and reproduction of the organism.
- Primary metabolites are typically formed during the growth phase as a result of energy metabolism, and are deemed essential for proper growth.
- Secondary metabolites do not play a role in growth, development, and reproduction like primary metabolites do, and are typically formed during the end or near the stationary phase of growth.