Enriched media
(noun)
Contains nutrients required to support the growth of a wide variety of organisms.
Examples of Enriched media in the following topics:
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Enrichment and Isolation
- Understanding the nutritional requirements of bacteria can aid their enrichment and isolation.
- An important distinction between growth media types is that of defined versus undefined media.
- Enriched media contain the nutrients required to support the growth of a wide variety of organisms, including some of the more fastidious ones.
- Blood agar is an enriched medium in which nutritionally-rich whole blood supplements the basic nutrients.
- Chocolate agar is enriched with heat-treated blood (40-45°C), which turns brown and gives the medium the color for which it is named.
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Generation Time
- However, exponential growth cannot continue indefinitely because the medium is soon depleted of nutrients and enriched with wastes.
- It emphasizes clonality, asexual binary division, the short development time relative to replication itself, the seemingly low death rate, the need to move from a dormant state to a reproductive state or to condition the media, and finally, the tendency of lab adapted strains to exhaust their nutrients.
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Selective and Differential Media
- Selective media allows for the growth of specific organisms, while differential media is used to distinguish one organism from another.
- Two types of media with similar implying names but very different functions, referred to as selective and differential media, are defined as follows.
- Some examples of selective media include:
- Differential media or indicator media distinguish one microorganism type from another growing on the same media.
- Examples of differential media include:
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Complex and Synthetic Media
- In defined media all the chemical compounds are known, while undefined media has partially unknown chemical constituents.
- There are many types of culture media, which is food that microbes can live on.
- Two major sub types of media are complex and synthetic medias, known as undefined and defined media.
- Undefined media are sometimes chosen based on price and sometimes by necessity - some microorganisms have never been cultured on defined media.A defined medium (also known as chemically defined medium or synthetic medium) is a medium in which all the chemicals used are known, no yeast, animal, or plant tissue is present.
- The term 'chemically defined media' is often misused in the literature to refer to serum albumin-containing media.
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Culture Media
- Culture media is the food used to grow and control microbes.
- These are the most common growth media, although specialized media are sometimes required for microorganism and cell culture growth.
- This is an important distinction between growth media types.
- Undefined media are sometimes chosen based on price and sometimes by necessity - some microorganisms have never been cultured on defined media.
- Differential media - Also known as indicator media, are used to distinguish one microorganism type from another growing on the same media.
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Whole-Genome DNA-Binding Analysis
- DNA bound by the protein will be coprecipitated and enriched, compared to DNA not bound by the respective protein.
- The two differentially-labeled DNAs are hybridized to the same microarray and the difference in fluorescence intensity gives a measure of the enrichment .
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Otitis Media
- Otitis media, or earache, is the inflammation of the middle ear and is often due to bacterial infections.
- Otitis media is inflammation of the middle ear .
- Though painful, otitis media is not threatening and usually heals on its own within 2–6 weeks.
- Typically, acute otitis media follows a cold.
- Otitis media is most commonly caused by infection with viral, bacterial, or fungal pathogens.
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Group B Streptococcus Colonization
- Sometimes, before plating, enrichment of the gathered probe is performed.
- The method of enrichment followed by the CAMP tests is currently the gold standard for GBS diagnosis.
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Detecting Acid and Gas Production
- Culture media can be used to differentiate between different kinds of bacteria by detecting acid or gas production.
- A microbiological culture, or microbial culture, is created using a method for multiplying microbial organisms by letting them reproduce in predetermined culture media under controlled laboratory conditions.
- Differential media, also known as indicator media, distinguish one microorganism type from another growing on the same media.
- Differential media are used for the detection of microorganisms and by molecular biologists to detect recombinant strains of bacteria.
- When cultured on an EMB (eosin methylene blue) plate, a positive result for E. coli is metallic green colonies on a dark purple media.
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Fermented Foods
- Biological enrichment of food substrates with protein, essential amino acids, essential fatty acids, and vitamins.