spectrophotometry

(noun)

A spectrophotometer is commonly used for the measurement of transmittance or reflectance of solutions. However they can also be designed to measure the diffusivity on any of the listed light ranges that usually cover around 200nm - 2500nm using different controls and calibrations. [2] Within these ranges of light, calibrations are needed on the machine using standards that vary in type depending on the wavelength of the photometric determination. [3]

Related Terms

Examples of spectrophotometry in the following topics:

  • Measurements of Microbial Mass

    • Using spectrophotometry for measuring the turbidity of cultures is known as turbidometry.
    • In spectrophotometry, cultures usually do not need to be diluted, although above a certain cell density the results lose reliability.
    • This has made spectrophotometry the methods of choice for quick measurements of bacterial growth and related applications.

  • Crystallographic Analysis

    • .), purification of recombinant proteins (such as chromatography of affinity and gel filtration), enzymatic tests and inhibition measurement (spectrophotometry), crystallization, x-rays crystallography and structural analysis, interactions determination (microcalorimetry, fluorescence, BIAcore), conformational analyses (circular dichroism, ultracentrifugation, light scattering), modifications analysis (mass spectrometry), bioinformatics, and molecular modelisation.

  • Complex Ion Equilibria and Solubility

    • To find Kc for this reaction experimentally, spectrophotometry can be used to measure the appearance of red color—indicating the forming [FeSCN]2+ ion.