radiolabelled

Examples of radiolabelled in the following topics:

• Primer Extension Analysis

  • This technique requires a radiolabelled primer (usually 20 to 50 nucleotides in length) which is complementary to a region near the 3' end of the gene.
  • By running the product on a polyacrylamide gel, it is possible to determine the transcriptional start site, as the length of the sequence on the gel represents the distance from the start site to the radiolabelled primer.

• DNA Sequencing Based on Sanger Dideoxynucleotides

  • Technical variations of chain-termination sequencing include tagging with nucleotides containing radioactive phosphorus for radiolabelling, or using a primer labeled at the 5' end with a fluorescent dye.