dideoxynucleotide

(noun)

Any nucleotide formed from a deoxynucleotide by loss of an a second hydroxy group from the deoxyribose group

Related Terms

Examples of dideoxynucleotide in the following topics:

  • DNA Sequencing Based on Sanger Dideoxynucleotides

    • This method is based on amplification of the DNA fragment to be sequenced by DNA polymerase and incorporation of modified nucleotides - specifically, dideoxynucleotides (ddNTPs).
    • To each reaction is added only one of the four dideoxynucleotides (ddATP, ddGTP, ddCTP, or ddTTP).
    • In dye-terminator sequencing, each of the four dideoxynucleotide chain terminators is labelled with fluorescent dyes, each of which emit light at different wavelengths .
    • Different types of Sanger sequencing, all of which depend on the sequence being stopped by a terminating dideoxynucleotide (black bars).