shotgun wedding

Examples of shotgun wedding in the following topics:

• Mate Selection

  • In a shotgun wedding, a marriage between two people is forced because of an unplanned pregnancy.
  • As the stigma associated with out-of-wedlock births has faded over the years, and the number of such births has increased, shotgun weddings have become less common.
  • Fewer people perceive shotgun weddings to be necessary in order to support the woman and the child.

• Strategies Used in Sequencing Projects

  • The strategies used for sequencing genomes include the Sanger method, shotgun sequencing, pairwise end, and next-generation sequencing.
  • In the shotgun sequencing method, several copies of a DNA fragment are cut randomly into many smaller pieces (somewhat like what happens to a round shot cartridge when fired from a shotgun).
  • This is the principle behind reconstructing entire DNA sequences using shotgun sequencing.
  • Originally, shotgun sequencing only analyzed one end of each fragment for overlaps.
  • Compare the different strategies used for whole-genome sequencing:  Sanger method, shotgun sequencing, pairwise-end sequencing, and next-generation sequencing

• Metagenomics

  • Recent studies use "shotgun" Sanger sequencing or massively parallel pyrosequencing to get largely unbiased samples of all genes from all the members of the sampled communities.
  • Advances in bioinformatics, refinements of DNA amplification, and the proliferation of computational power have greatly aided the analysis of DNA sequences recovered from environmental samples, This allows the adaptation of shotgun sequencing to metagenomic samples.
  • Shotgun sequencing and screens of clone libraries reveal genes present in environmental samples.
  • Shotgun metagenomics is also capable of sequencing nearly complete microbial genomes directly from the environment.
  • On the other hand, the random nature of shotgun sequencing ensures that many of these organisms, which would otherwise go unnoticed using traditional culturing techniques, will be represented by at least some small sequence segments.

• Detecting Uncultured Microorganisms

  • These advances have allowed the adaptation of shotgun sequencing to metagenomic samples .
  • Shotgun sequencing and screens of clone libraries reveal genes present in environmental samples.
  • This was further followed by high-throughput sequencing which did the same process as the shotgun sequencing but at a much bigger scale in terms of the amount of DNA that could sequenced from one sample.

• DNA Sequencing Techniques

  • Most genomic sequencing projects today make use of an approach called whole genome shotgun sequencing.
  • Whole genome shotgun sequencing involves isolating many copies of the chromosomal DNA of interest.
  • In shotgun sequencing, multiple copies of the same chromosome are isolated and then fragmented in random locations.

• Genetic Analysis

  • There are two main approaches to sequencing microbial genomes – the ordered clone approach and direct shotgun sequencing both require large and small insert genomic DNA libraries in order to be effective.
  • Summarize the techniques used to study genomes: PFGE. ordered clone approach, direct shotgun sequencing and microarray hybridization

• Speeches for Special Occasions

  • Clearly this speech will be very different than a toast given at a wedding, which is also an example of a special occasion speech and which will have a much more celebratory tone.
  • For example, when someone gives a speech at a wedding and shares a specific memory, thus informing the audience of that particular incident and possibly articulating its importance in relation to the newlyweds.
  • The toasts that are given at a wedding are an example of special occasion speeches.

• Marriage and Responsibility

  • The ceremony in which a marriage is enacted and announced to the community is called a wedding.
  • A marriage can be declared by a wedding ceremony, which may be performed either by a religious officiator or through a similar government-sanctioned secular process.
  • In many countries, heterosexual weddings have the women dress in traditional white with a veil and the men in a tuxedo.

• Practical Tips for Speaking in Non-Academic Settings

  • Entertainment speeches may be given at a wedding or other social event.
  • The toasts that are given at a wedding are an example of special occasion speeches.

• Situational and Environmental Context

  • A best man gives a toast at a wedding.
  • A politician wouldn't knight Sir Ian nor would a best man give a stump speech at a wedding.
  • On a lighter note, you might be at your best friend's wedding and asked to give one of the first toasts.
  • For example, you wouldn't read a eulogy at a wedding?