pyrosequencing

Examples of pyrosequencing in the following topics:

• Metagenomics

  • Recent studies use "shotgun" Sanger sequencing or massively parallel pyrosequencing to get largely unbiased samples of all genes from all the members of the sampled communities.
  • The first metagenomic studies conducted using high-throughput sequencing used massively parallel 454 pyrosequencing.
  • These techniques for sequencing DNA generate shorter fragments than Sanger sequencing; 454 pyrosequencing typically produces ~400 bp reads, Illumina and SOLiD produce 25-75 bp reads.
  • Pyrosequenced metagenomes generate 200–500 megabases, while Illumina platforms generate around 20–50 gigabases.

• DNA Sequencing Techniques

  • The most popular, widely-used second-generation sequencing method was one called Pyrosequencing.