Kreb's cycle

Examples of Kreb's cycle in the following topics:

• Citric Acid and Other Organic Compounds

  • Citric acid (citrate) is an important substance in the Krebs cycle.
  • The Krebs cycle is key in the oxidation of sugars, proteins and fats to carbon dioxide and water.
  • Many of the cycle compounds are also needed for the synthesis of the cells' own proteins, carbohydrates, and fats.
  • The microorganism makes more citric acid in the Krebs cycle than needed for the cell's metabolism and exports it outside the cell.

• Pyruvic Acid and Metabolism

  • Pyruvic acid supplies energy to living cells through the citric acid cycle (also known as the Krebs cycle) when oxygen is present (aerobic respiration); when oxygen is lacking, it ferments to produce lactic acid.
  • Pyruvate is converted into acetyl-coenzyme A, which is the main input for a series of reactions known as the Krebs cycle.
  • Pyruvate is also converted to oxaloacetate by an anaplerotic reaction, which replenishes Krebs cycle intermediates; also, oxaloacetate is used for gluconeogenesis.
  • The cycle is also known as the citric acid cycle or tri-carboxylic acid cycle, because citric acid is one of the intermediate compounds formed during the reactions.
  • Pyruvic acid supplies energy to living cells through the citric acid cycle (also known as the Krebs cycle) when oxygen is present (aerobic respiration), and alternatively ferments to produce lactic acid when oxygen is lacking (fermentation).

• Importance of Glycolysis

  • Following the conversion of glucose to pyruvate, the glycolytic pathway is linked to the Krebs Cycle, where further ATP will be produced for the cell's energy needs.
  • It is followed by the Krebs cycle and oxidative phosphorylation to produce ATP.

• Respiration and Proton Motive Force

  • During aerobic conditions, the pyruvate enters the mitochondrion to be fully oxidized by the Krebs cycle.
  • The products of the Krebs cycle include energy in the form of ATP (via substrate level phosphorylation), NADH, and FADH2.
  • Both types of metabolism share the initial pathway of glycolysis, but aerobic metabolism continues with the Krebs cycle and oxidative phosphorylation.
  • A diagram of cellular respiration including glycolysis, Krebs cycle (AKA citric acid cycle), and the electron transport chain.

• Acetyl CoA and the Citric Acid Cycle

  • The citric acid cycle, shown in —also known as the tricarboxylic acid cycle (TCA cycle) or the Krebs cycle—is a series of chemical reactions used by all aerobic organisms to generate energy through the oxidation of acetate—derived from carbohydrates, fats, and proteins—into carbon dioxide.
  • The NADH generated by the TCA cycle is fed into the oxidative phosphorylation pathway.
  • Components of the TCA cycle were derived from anaerobic bacteria, and the TCA cycle itself may have evolved more than once.
  • Theoretically there are several alternatives to the TCA cycle, however the TCA cycle appears to be the most efficient.
  • The citric acid cycle, or Krebs cycle, is a series of chemical reactions used by all aerobic organisms to generate energy through the oxidization of acetate—derived from carbohydrates, fats, and proteins—into carbon dioxide.

• The Reverse TCA Cycle

  • The reverse TCA cycle utilizes carbon dioxide and water to form carbon compounds.
  • The citric acid cycle (TCA) or Krebs cycle, is a process utilized by numerous organisms to generate energy via the oxidation of acetate derived from carbohydrates, fats, and proteins into carbon dioxide .
  • However, there are numerous organisms that undergo reverse TCA or reverse Krebs cycles.
  • The chemical reactions that occur are the reverse of what is seen in the TCA cycle .
  • The following is a brief overview of the reverse TCA cycle.

• Absorptive State

  • The liver deaminates amino acids to keto acids to be used in the Kreb's cycle in order to generate energy in the form of ATP.

• Acetyl CoA to CO2

  • The acetyl carbons of acetyl CoA are released as carbon dioxide in the citric acid cycle.
  • Acetyl CoA links glycolysis and pyruvate oxidation with the citric acid cycle.
  • In addition to the citric acid cycle, named for the first intermediate formed, citric acid, or citrate, when acetate joins to the oxaloacetate, the cycle is also known by two other names.
  • Additionally, the cycle is known as the Krebs cycle, named after Hans Krebs, who first identified the steps in the pathway in the 1930s in pigeon flight muscle.
  • Describe the fate of the acetyl CoA carbons in the citric acid cycle

• Citric Acid Cycle

  • The last step in the citric acid cycle regenerates oxaloacetate by oxidizing malate.
  • Each turn of the cycle forms three NADH molecules and one FADH2 molecule.
  • One GTP or ATP is also made in each cycle.
  • Several of the intermediate compounds in the citric acid cycle can be used in synthesizing non-essential amino acids; therefore, the cycle is amphibolic (both catabolic and anabolic).
  • Because the final product of the citric acid cycle is also the first reactant, the cycle runs continuously in the presence of sufficient reactants.

• Homeostatic Responses to Shock

  • Since oxygen is not abundant, the Kreb's cycle is slowed resulting in lactic acidosis (the accumulation of lactate).